PHARMACOGNOSTIC AND ANTIBACTERIAL STUDIES OF ACACIA SIEBERIANA VAR WOODII (FABACEAE) STEM BARK

PHARMACOGNOSTIC AND ANTIBACTERIAL STUDIES OF ACACIA SIEBERIANA VAR WOODII (FABACEAE) STEM BARK

ABSTRACT

Acacia sieberiana var. Woodii (Fabaceae) is traditionally used as a remedy for stomach aches and ulcers among some localities in Northern Nigeria. The plant was investigated sequel to the reports of its ethnomedicine uses in the management of stomach ache and ulcers. Anatomical and physical constants studies were carried out using standard procedures. Phytochemical (preliminary studies, thin layer and column chromatography) were also carried out on the hexane and methanol extracts. Toxicity and antibacterial studies of the hexane and methanol extracts, and the isolated compound were investigated. The microscopical studies revealed the presence of cellulose cell wall, lignified and suberized cell walls, tannins and calcium crystals. Anatomical features identified in the stem bark consist of epidermis, phellogen, phelloderm, and cortex. The percentage moisture content and ash values were observed to be 9.0% and 10.5% respectively. Water extractive value was 1.2% while ethanol extractive value was 1.6%.The preliminary phytochemical studies as well as the TLC chromatogram of the hexane extract revealed the presence of steroids and triterpenes while the methanol extract contains tannins, flavonoids, alkaloids, steroids and triterpenes. Fraction AS1collected through column chromatography was suggested to be a hydrocarbon on the basis of its colourless and oily nature. The acute toxicity study carried out indicated that the hexane extract was slightly toxic while the methanol extract was moderately toxic. Antibacterial studies of the stem bark carried out showed zones of inhibition between 16 to 27 mm for the hexane and methanol extracts against Helicobacter pylori, Escherichia coli, Salmonella typhi and Shigella dysenteriae. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were between the range of 2.5 and 10 mg/ml and 5.0 to viii 20 mg/ml respectively. Fraction AS1 exerted a dimension of zones of inhibition between 26 to 30 mm against all the bacterial strains except S. typhi. It was also able to exert MIC and MBC against E. coli and H. pylori at 12.5 and 25µg/ml respectively, while it had MIC and MBC at the lowest concentrations of 6.25 and 12.5 µg/ml against S. dysenteriae. Results of the present studies had shown that A. sieberiana and fraction AS1 collected from this plant have wide antibacterial property.

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